As the immune system battles pneumonia, the outcome of that war may hinge on a microscopic arms race over iron.
Scientists say they have figured out how that race for iron actually increases the risk we face from one of our most dangerous microscopic foes, Klebsiella pneumoniae. They made the discovery in mice with pneumonia.
K. pneumoniae has already figured out how to overcome our best defenses—including, in some cases, all our most powerful antibiotics.
It’s the third-most-common cause of infections that arise in hospitalized patients, and causes pneumonia, urinary tract infections, wound infections, and bloodstream infections. In its most drug-resistant form, it’s considered one of the carbapenem resistant Enterobacteriaceae, or CRE—so called “nightmare bacteria”.
The new findings could aid the search for drugs to fight it, and other “superbugs.”
In a new paper in the journal mBio, a team led by Michael Bachman at the University of Michigan Medical School reports what happens after the bacteria send out tiny iron-scavenging molecules.
Called siderophores, those molecules have long been thought of as a way for bacteria to gather a precious element needed to grow and reproduce, by attaching to iron and stealing it from us.
Siderophores from K. pneumoniae are hundreds of times more powerful at grabbing iron than the proteins that our own bodies produce. What’s more, the bacteria produce a kind of siderophore that our defense systems can’t neutralize.
But Bachman and his colleagues show that bacterial siderophores do much more than just grab iron. Their experiments show that K. pneumoniae actually use the molecules to help them invade the rest of the body beyond their initial point of entry, and to bring on inflammation caused by our own immune system.
“This is a bacterium that has evolved new ways to get iron, and it turns out that the mechanism it uses also causes cellular stress during infections,” says Bachman, an assistant professor of pathology. “That response triggers an immune response that tells our bodies to fight the infection, but it also activates a mechanism that allows bacteria to escape and travel to the rest of the body.”
That mechanism, a protein called Hif-1alpha, normally helps our bodies respond to low oxygen or low iron. But when K. pneumoniae siderophores activate it, it worsens the infection. Exactly how is still a mystery.
Bachman notes that it’s too early to say exactly how the discovery could be used to help patients.
One promising route could be to develop strategies to prevent the bacteria from sending out siderophores in the first place—or to use the siderophores to bring antibiotics back into bacterial cells. Or, it may even be possible to create a vaccine based on siderophores to teach the immune system to attack them as invaders.
Meanwhile, he and colleagues are pursuing a broader goal through the University of Michigan’s Host Microbiome Initiative. Using an advanced genetic technique called transposon sequencing, they’re working to figure out which genes K. pneumoniae and other superbugs absolutely need in order to cause infections.
To figure out the double role that siderophores play in a K. pneumoniae infection, Bachman and his colleagues had to figure out how to create bacteria that would not reproduce, while still producing siderophores. They were able to mutate bacteria in a way that kept iron-laden siderophores from getting back into the bacterial cells and promoting growth. That way, the number of bacteria in a mouse’s body stayed the same, but the researchers could study what the siderophores did.
They expected that if the siderophores grabbed enough iron from the mouse’s lung tissue and robbed the mouse an essential element, the mouse’s cells would respond. Indeed, they found that the mouse immune system was triggered directly by the siderophores, causing the release of molecules called cytokines that attract infection-fighting cells and cause inflammation as a result. The activation of Hif-1alpha was part of this–because the mouse cells sensed that iron supply was low.
So, the team figured out how to eliminate Hif-1alpha in the cells lining the mouse lungs—and showed that in these mice, most of the bacteria couldn’t escape from the lungs to the spleen. But in mice that made Hif-1alpha, more K. pneumoniae bacteria spread to the rest of the body, making the infection worse.
“This work provides us with motivation to target the production of siderophores, rather than just the uptake of them,” says Bachman. “Now that we know that the bacteria cause cellular stress just by secreting them, we may be able to prevent these effects if we neutralize them.”
Funding for the work came from the Natural Sciences and Engineering Research Council of Canada, through a grant to coauthor Martin Dozois at the Institut national de la recherche scientifique, Institut Armand-Frappier, in Laval, Quebec.
Source: University of Michigan
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