A new study complicates the “five-second rule,” the widely accepted idea that it’s okay to scoop up fallen food and eat it if you’re quick enough.
Donald Schaffner, professor and extension specialist in food science at Rutgers University, found that moisture, type of surface, and contact time all contribute to cross-contamination. In some instances, the transfer begins in less than one second.
“The popular notion of the ‘five-second rule’ is that food dropped on the floor, but picked up quickly, is safe to eat because bacteria need time to transfer,” Schaffner says, adding that while the pop culture “rule” has been featured by at least two TV programs, research in peer-reviewed journals is limited.
“We decided to look into this because the practice is so widespread. The topic might appear ‘light’ but we wanted our results backed by solid science,” says Schaffner, who conducted research with Robyn Miranda, a graduate student in his laboratory at the School of Environmental and Biological Sciences at Rutgers University-New Brunswick.
The researchers tested four surfaces—stainless steel, ceramic tile, wood, and carpet—and four different foods (watermelon, bread, bread and butter, and gummy candy). They also looked at four different contact times—less than one second, five, 30, and 300 seconds. They used two media—tryptic soy broth or peptone buffer—to grow Enterobacter aerogenes, a nonpathogenic “cousin” of Salmonella that naturally occurs in the human digestive system.
Transfer scenarios were evaluated for each surface type, food type, contact time, and bacterial prep; surfaces were inoculated with bacteria and allowed to completely dry before food samples were dropped and left to remain for specified periods. All totaled 128 scenarios were replicated 20 times each, yielding 2,560 measurements. Post-transfer surface and food samples were analyzed for contamination.
Not surprisingly, watermelon had the most contamination, gummy candy the least. “Transfer of bacteria from surfaces to food appears to be affected most by moisture,” Schaffner says. “Bacteria don’t have legs, they move with the moisture, and the wetter the food, the higher the risk of transfer. Also, longer food contact times usually result in the transfer of more bacteria from each surface to food.”
Perhaps unexpectedly, carpet has very low transfer rates compared with those of tile and stainless steel, whereas transfer from wood is more variable. “The topography of the surface and food seem to play an important role in bacterial transfer,” Schaffner says.
So while the researchers demonstrate that the five-second rule is “real” in the sense that longer contact time results in more bacterial transfer, it also shows other factors, including the nature of the food and the surface it falls on, are of equal or greater importance.
“The five-second rule is a significant oversimplification of what actually happens when bacteria transfer from a surface to food,” Schaffner says. “Bacteria can contaminate instantaneously.”
The findings appear online in the journal Applied and Environmental Microbiology.
Source: Rutgers University