Thermos device quickly detects Zika for only $2

Engineers have developed a rapid, low-cost genetic test for Zika. The $2 device, about the size of a soda can, doesn’t require electricity or technical expertise and only needs a bit of saliva.

Color-changing paper in the lid turns blue if the genetic assay chip inside detects the virus.

Rapid, accurate diagnosis is especially important for pregnant women who may be infected, researchers say. However, the only approved tests for the virus currently require highly sensitive laboratory equipment. Diagnostic tools that can be used in the field, while the patient waits, would be a critical tool for fighting the Zika epidemic.

Zika test cut-away
(Credit: Penn)
Zika test
(Credit: Penn)

Assays that detect genetic material from the Zika virus itself are considered the gold standard in diagnostics. Alternatives, such as tests that look for antibodies the body creates in response to the virus, are insufficient as they may produce false negatives from people who are infected but haven’t yet produced enough antibodies, or false positives from people who have antibodies for a different disease that is similar enough to trigger the test.

This cheap test diagnoses Zika in hours

Tests that look for RNA sequences from the virus itself, known as reverse transcriptase polymerase chain reaction, or RT-PCR, avoid both problems. However, RT-PCR requires delicate laboratory work. Viral gene sequences in a patient’s sample must be amplified, or repeatedly copied, to levels where they can be detected, a process that normally involves multiple precise temperature changes.

“The CDC has approved, on an emergency basis, only these kinds of laboratory-based molecular tests for the Zika virus,” says Changchun Liu, research assistant professor of mechanical engineering and applied mechanics at the University of Pennsylvania.

“Generally, lateral flow tests, which directly change the color of a test strip based on the presence of Zika antibodies, suffer from low sensitivity. And since antibodies to the Zika virus cross-react with other similar viruses prevalent in Zika-endemic areas, lateral flow tests for Zika also suffer from low specificity.”

With the amplification step as the main hurdle to a portable genetic test, researchers investigated the possibility of using an alternative technique known as LAMP, or loop-mediated isothermal amplification, which only requires the sample to be kept at a specific temperature, not cycled through multiple precise temperature changes as in RT-PCR.

40-minute test

As a trade-off for this simplified amplification process, LAMP requires even more specialized “primers,” short gene sequences that are designed to match the regions of the virus’ DNA targeted by the test.

“Although Zika primers for RT-PCR have been published in the literature, LAMP primers have not,” says Haim Bau, professor of mechanical engineering and applied mechanics. “So, using data mining, we identified highly conserved regions of the Zika virus genome that are divergent from other known pathogens. We then designed appropriate primers to recognize this sequence.

“In parallel, we engineered a low-cost, point-of-care system that consists of a diagnostic cassette and a processor. The cassette isolates, concentrates, and purifies nucleic acids and carries out enzymatic amplification. The test results are indicated by the change in the color of a dye, which can be inspected visually.”

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The researchers then worked on how to keep the sample at the necessary temperature without using electricity. Their solution involved a thermos bottle, a self-contained heating element that uses a chemical reaction from portable military rations and a wax-like material that absorbs excess heat by melting. A 3D printed lid fits on top of the thermos and holds all of the test’s components in place.

Once a patient’s saliva sample is introduced into the cartridge, the test takes about 40 minutes to run. The researchers demonstrated its efficacy with their own saliva spiked with virus samples, showing sensitivity equivalent to that of RT-PCR tests. Future work will demonstrate the test’s selectivity and will also test a version that can quantify the viral load by means of a fluorescent dye and an integrated smartphone camera.

“Our work represents a proof of concept at this stage,” Bau says. “Before the assay can be adapted for medical use, we must experiment with patients’ samples and make sure that our assay and system match the performance of the gold standard and operate reproducibly and reliably.”

The National Institutes of Health and the Penn Center for AIDS Research funded the work.

Source: University of Pennsylvania

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Source: Futurity