These neurons let us wake up in the middle of the night

Researchers have identified a group of neurons that may keep us awake even when we want to sleep.

The findings, from research with mice, have implications for treating insomnia, oversleeping, and sleep disturbances that accompany other neuropsychiatric disorders such as depression.

Biologist Viviana Gradinaru and her team wanted to know: How do we overcome tiredness in the face of a looming deadline or rouse ourselves in the dead of night to feed a crying baby? In other words, in the face of so-called salient stimuli, how do we override the natural drive to sleep?

DRNDA neurons
Dopaminergic neurons genetically engineered to express fluorescing genes. The fluorescence is used to measure activity of the neurons, which have been implicated in the sleep/wake cycle. (Credit: Gradinaru laboratory/UC Santa Barbara)

“To answer this question, we decided to examine a region of the brain, called the dorsal raphe nucleus, where there are an under-studied group of dopamine neurons called dorsal raphe nucleus neurons, or DRNDA neurons,” says Gradinaru, an assistant professor of biology and biological engineering and director of the Center for Molecular and Cellular Neuroscience of the Tianqiao and Chrissy Chen Institute for Neuroscience at the California Institute of Technology.

“People who have damage in this part of their brain have been shown to experience excessive daytime sleepiness, but there was not a good understanding of the exact role of these neurons in the sleep/wake cycle and whether they react to internal or external stimuli to influence arousal,” she says.

The team studied DRNDA neurons in mice, which are a model organism for studying the human brain. First, the team measured DRNDA activity while the animals encountered salient stimuli, such as the arrival of a potential mating partner, a sudden unpleasant sensation, or food.

The DRNDA neurons were highly active during these events, which led the researchers to theorize that the neurons send signals of salience and arousal, which can then modulate the state of sleep or wakefulness.

“We then measured DRNDA activity throughout the sleep/wake cycle and found that these neurons are least active when the animal is sleeping and increase in activity as the animal is waking up,” says Ryan Cho, a graduate student and the first author of the paper.

“We aimed to discover whether this was just a correlation or if the activity of the neurons was actually causing changes in sleep-wake states,” he says.

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The researchers used a technique called optogenetics to engineer DRNDA cells to be stimulated by light. After stimulating these neurons with light during the time that the animal would normally sleep, Gradinaru and her team found that the mouse woke up from sleep and remained awake.

The reverse was true when the activity of DRNDA was chemically silenced—the animal was likely to fall asleep, even in the face of motivationally important stimuli, such as the odor of a predator or a mating partner. This implied that activity of the DRNDA neurons truly governed sleep-wake behaviors.

Finally, the researchers examined the role of these neurons in awaking due to external stimuli. The neurons’ activity was silenced with optogenetics, and a loud noise was played while the animals were asleep. Whereas control mice often woke up, the mice with blocked DRNDA often ignored the sound and remained asleep.

“These experiments showed us that DRNDA cells are necessary for full wakefulness in the face of important stimuli in mice,” Gradinaru says. “DRNDA neurons are found analogously in humans, and while they have not been studied in depth, their degeneration has been correlated with excessive daytime sleepiness in patients with neurodegenerative disorders such as multiple systems atrophy and Lewy body dementia.

“Further work is necessary to establish causation in humans and to test the potential of the DRNDA as a therapeutic target for insomnia or oversleeping, and for sleep disturbances that accompany other psychiatric disorders such as depression, bipolar disorder, and schizophrenia,” Gradinaru adds.

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The study appears in the journal Neuron. The National Institutes of Health, the NIH’s National Institute on Aging, the Heritage Medical Research Institute, the Pew Charitable Trusts, the Michael J. Fox Foundation, the Caltech-Gwangju Institute of Science and Technology exchange program, and the Alfred P. Sloan Foundation provided funding for the work.

Source: California Institute of Technology

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